Small RNA Library Prep Kit for Illumina Methylation Bisulfite Hotstart Taq has 5'-3' polymerase

Hotstart Taq has 5'-3' polymerase activity

salts and other contaminants

but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→5´ exonuclease activity

and heparin

- Purification of PCR products: nucleotide degradation of PCR products before sequencing

Small RNA Library Prep Kit for Illumina Methylation Bisulfite Hotstart Taq has 5'-3' polymeraseThe Small RNA Library Prep Kit for Illumina is designed to construct small RNA sequencing libraries for Illumina platforms. It ligates universal adaptors to the 3' and 5' ends of small RNAs, followed by reverse transcription, PCR amplification, and purification via PAGE gel or magnetic beads. It supports total RNA inputs from 10 ng to 1 g, as well as purified serum, plasma, and exosome RNA (1 ng). Strict quality control ensures consistent library