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The addition of a carbon source is required
05 L-Lysine HCl 0
2 at 25°C Suspend 70 gram powdered medium in 1 litre distilled water
a transformation of proteins into peptides
Ferric chloride
Vegetal LoFlo Medium Size:1000g The addition of a carbonLoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long term culturing. Typically, cells are grown only overnight in LoFlo, and transferred to fresh Lo Flo the following morning prior to imaging, so that LoFlo growth limited to
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