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2 at 25°C Suspend 30 gram powdered medium in 1 litre distilled water
Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen
Gentamicin is an aminoglycoside antibioticum with bactericidal activity against many gram-negative bacteria
Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins
15 (NH4)6M07O24
SIH Medium Size:250g 2 at 25°C Suspend 30SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic
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