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Water and soil quality. Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of <i>Caenorhabditis elegans</i> (Nematoda) Ingestion-build-266356 This document provides guiding principles1 Scope This document specifies a method for determining the toxicity of environmental samples on growth, fertility and reproduction of Caenorhabditis elegans. The method applies to contaminated whole freshwater sediment (maximum salinity 5 g l), soil and waste, as well as to pore water, elutriates and aqueous extracts that were obtained from contaminated sediment, soil and waste.