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LBM-Broth Miller Size:1000g Molecular cloning: a laboratory manual

SKU: 82403522152

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Description

Molecular cloning: a laboratory manual

Dilute 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer

Cold Spring Harbour laboratory

In LBM media Mg2 is added to enhance absorption of phage lambda to the cells

10 pH (100 g/l) 2

LBM-Broth Miller Size:1000g Molecular cloning: a laboratory manualLBM Broth High Salt In LBM media Mg2 is added to enhance adsorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 10 MgSO4. anhydrous 0. 98 Final pH 7. 0 0. 2 at 25C Suspend 26 gram powdered medium in 1 litre distilled water Store dry at room temperature

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