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Final concentration is 40mM Tris Base
7 (10 g/l) Assay ≥99%
Tryptone 10 Yeast extract 5 NaCl 10 MgSO4
2 at 25°C Suspend 28 gram powdered medium in 1 litre distilled water
15 (NH4)6Mo7O24
LBM-Agar Lennox Size:1000g Final concentration is 40mM TrisLBM Agar Low Salt For propagation of of lambda phages in Escherichia coli. In LBM media Mg2 is added to enhance absorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 5 MgSO4 anhydrous 0. 98 Agar 15 Final pH 7. 0 0. 2 at 25C Suspend 36 gram powdered medium in 1 litre distilled water Store dry at room temperature
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