TATAA two-tailed miRNA assays miRNA Assay:miR-451a-5p (AAACCGUUACCAUUACUGAGUU) with each mix containing all

with each mix containing all 69 SIRV isoform transcripts (from 7 SIRV genes) but in different concentration ratios

The measured Cq-values and the shape of the amplification curves reflect the inhibition

or 15 min at 60°C

It degrades only double-stranded DNA (dsDNA) and leaves RNA and single-stranded DNA (ssDNA) intact

The TATAA EvaGreen® GrandMaster® Mix provides a robust and versatile solution for ultimate sensitivity and efficiency

TATAA two-tailed miRNA assays miRNA Assay:miR-451a-5p (AAACCGUUACCAUUACUGAGUU) with each mix containing allTATAA two tailed RT qPCR microRNA assays Our new TATAA miRNA two Tailed RT qPCR assays show exceeding performance compared to other techniques. The challenge detecting small microRNAs is that two conventional PCR primers do not fit the target as their combined length is almost twice the length of the microRNA. Older techniques have solved this by extending the microRNA using eg., a hairpin primer, adding a poly A tail, or adding a fragment by